rabbit polyclonal antibodies cd117  (Bioss)

Journal: PLOS ONE

Article Title: Involvement of an IgE/Mast cell/B cell amplification loop in abdominal aortic aneurysm progression

doi: 10.1371/journal.pone.0295408

Figure Lengend Snippet: (A) Cross-sections of AAAs were coloured with Perl’s+DAB staining and nucleus were counter-stained with nuclear fast red. TLOs (circled with dashed line) are found at proximity of haematomas (arrowheads). Scale bar: 500 μm. (B) Consecutive sections were stained for CD20 (magenta), IgE (green), CD117 (red) and DAPI (grey). B cells (CD20 + and DAPI + ) appear in light pink, autofluorescent red blood cells (low intensity in the CD20 channel and DAPI - ) appear in magenta, and MCs (CD117 + IgE + ) appear in yellow. Scale bar: 250 μm. (C) Magnified inset highlighting the close proximity of IgE + TLOs (dashed circle), red blood cells (white arrowheads) and MCs (blue arrows). (D) Magnified inset highlighting MCs (blue arrows) at proximity to intramural haematoma. (E-F) Adventitia from NAA organ donors and AAA patients were digested and analysed by flow cytometry after the addition of fluorescent count beads. Singlet, not autofluorescent, live CD45 + cells were selected, and MCs were identified as CD3 - CD19 - FcεRIa + CD117 + cells (E). The number of MCs (F, left) and activated (CD107a hi ) MCs (F, right) was calculated in each sample. Error bars represent mean +/- standard error; Mann-Whitney test (***, p < 0.001). (G-H) Cross-sections of AAAs were stained for CD20 (magenta), IgE (green), and tryptase (red). MCs (tryptase + IgE + ) were detected around TLOs. Scale bar: 100 μm. The magnified inset (H) shows a degranulating tryptase + MC at proximity of a TLO.

Article Snippet: Alternatively, sections were incubated with retrieval reagent (R&D Systems), then immunostained using antibodies against IgE (goat polyclonal, Vector Laboratories), MC tryptase (rabbit monoclonal, clone EPR8476, abcam; or mouse monoclonal, clone AA1, abcam), CD20 (mouse monoclonal, clone L26, Dako; or goat polyclonal, Thermofisher), CD117 (rabbit polyclonal, Dako), or glycophorin A (rabbit monoclonal, clone EPR8200, abcam).

Techniques: Staining, Flow Cytometry, MANN-WHITNEY

Journal: Revista da Associação Médica Brasileira

Article Title: Does resveratrol reduce cisplatin-induced ovarian damage?

doi: 10.1590/1806-9282.20230314

Figure Lengend Snippet: Light microscopic appearance of ovary (A–C) and immunohistochemical staining of rats by using c-kit (D–F) . (A) Minimally fibrosis and congestion in the ovarian stroma of the rat from the control group (H&E, ×50). (B) Lipoid cell storage (arrow), fibrosis, and lymphocyte infiltration in the ovarian stroma of the rat from the cisplatin group (H&E, ×100). (C) Mild fibrosis, congestion, and lymphocyte infiltration in the ovarian stroma of the rat from the cisplatin+resveratrol group (H&E, ×50). (D) Negative staining with c-kit (cd117) in the ovary of the rat from the control group (×100). (E) Focal 10% staining with c-kit (cd117) in the ovary of the rat from the cisplatin group (×100). (F) Focal 10% but less than cisplatin group staining with c-kit (cd117) in the ovary of the rat from the cisplatin + resveratrol group (×100).

Article Snippet: A polyclonal rabbit anti-human CD117 antibody (1:400; Dako, Glostrup, Denmark) was used to grade the immunohistochemical staining for c-kit expression.

Techniques: Immunohistochemical staining, Staining, Negative Staining

Journal: Arthritis Research & Therapy

Article Title: Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and –negative rheumatoid arthritis patients

doi: 10.1186/ar4372

Figure Lengend Snippet: Immunohistochemistry characteristics according to ACPA status

Article Snippet: The slides were subsequently stained with an automated immunostainer (TechMate 500 Plus; Dako, Cambridge, UK) using the following monoclonal antibodies: anti-CD3 (clone PS1; Novocastra, Newcastle, UK), anti-CD20 (clone L26; Dako), anti-CD15 (clone BY87; Novocastra), anti-CD31 (clone JC70A; Dako), anti-CD68 (clone KP-1; Dako), anti-CD117 (mast cells, rabbit anti-human polyclonal antibody; Dako).

Techniques: Immunohistochemistry